rat α2δ1 subunit (Addgene inc)

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Addgene inc rat α2δ1 subunit
Rat α2δ1 Subunit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+%CE%B12%CE%B41+subunit/rat+%CE%B12%CE%B41+subunit/pm33020266-303-30-32
Average 90 stars, based on 1 article reviews

rat α2δ1 subunit - by Bioz Stars, 2026-09

90/100 stars

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Patch Clamp:

Article Title: Zebrafish calls for reinterpretation for the roles of P/Q calcium channels in neuromuscular transmission
Article Snippet: .. Whole cell patch clamp recording of expressed calcium channels Human embryonic kidney (HEK293T) cells were transfected by Lipofectamine 2000 (Invitrogen) with a mixture containing, in equal molar ratio, cDNAs for the EGFP-tagged α subunit, rat α2δ1 subunit (Addgene, accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF286488","term_id":"11055591","term_text":"AF286488"}} AF286488 ) and the zebrafish β4b subunit. ..

Transfection:

Article Title: Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish
Article Snippet: .. HEK293T cells were transfected by Lipofectamine 2000 (Invitrogen) with complementary DNAs (cDNAs) for the zebrafish calcium channel α-subunit (accession no. {"type":"entrez-nucleotide","attrs":{"text":"KC192783","term_id":"540119135"}} KC192783 for the P/Q channel, {"type":"entrez-nucleotide","attrs":{"text":"KC192784","term_id":"540119137"}} KC192784 for the N type), rat α2δ1-subunit (Addgene; accession no. {"type":"entrez-nucleotide","attrs":{"text":"AF286488","term_id":"11055591"}} AF286488 ), and zebrafish β4b-subunit (accession no. {"type":"entrez-nucleotide","attrs":{"text":"KC192785","term_id":"540119139"}} KC192785 ), in equal molar ratio. ..

Article Title: Zebrafish calls for reinterpretation for the roles of P/Q calcium channels in neuromuscular transmission
Article Snippet: .. Human embryonic kidney (HEK293T) cells were transfected by Lipofectamine 2000 (Invitrogen) with a mixture containing, in equal molar ratio, cDNAs for the EGFP-tagged α subunit, rat α2δ1 subunit (Addgene, accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF286488","term_id":"11055591","term_text":"AF286488"}} AF286488 ) and the zebrafish β4b subunit. ..

Imaging:

Article Title: The Ras-like GTPase Rem2 is a potent inhibitor of calcium/calmodulin-dependent kinase II activity
Article Snippet: .. Calcium imaging HEK293T cells stably expressing the mouse Cav2.1 subunit (Addgene 26578), the rat α2δ1 subunit (Addgene 26575), and the rat β4 subunit (Addgene 107426) were created using the sleeping beauty transposase system ( 54 ). .. The transposase system plasmids used were pSBbi-GB (Addgene 60520 for Cav2.1), pSBbi-pur (Addgene 60523 for β4), and pSBbi-hyg (Addgene 60524 for α2δ1).

Stable Transfection:

Article Title: The Ras-like GTPase Rem2 is a potent inhibitor of calcium/calmodulin-dependent kinase II activity
Article Snippet: .. HEK293T cells stably expressing the mouse Cav2.1 subunit (Addgene 26578), the rat α2δ1 subunit (Addgene 26575), and the rat β4 subunit (Addgene 107426) were created using the sleeping beauty transposase system ( 54 ). .. The transposase system plasmids used were pSBbi-GB (Addgene 60520 for Cav2.1), pSBbi-pur (Addgene 60523 for β4), and pSBbi-hyg (Addgene 60524 for α2δ1).

Expressing:

other:

Article Title: Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish.
Article Snippet: HEK293T cells were transfected by Lipofectamine 2000 (Invitrogen) with complementary DNAs (cDNAs) for the zebrafish calcium channel α-subunit (accession no. KC192783 for the P/Q channel, KC192784 for the N type), rat α2δ1subunit (Addgene; accession no. AF286488), and zebrafish β4b-subunit (accession no. KC192785), in equal molar ratio.

Review